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Further mechanistic studies showed that NNMTi increases intracellular NAD levels and activates the SIRT1 pathway by inhibiting NNMT, thereby suppressing the transcription of adipogenic genes and preventing the maturation of preadipocytes.This research provides a new perspective for understanding the molecular mechanism of adipocyte differentiation, confirms the key role of NNMTi in regulating adipogenesis, and lays a foundation for subsequent obesity intervention studies

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Gut microbiota regulates depression-like behavior in rats through the neuroendocrine-immune-mitochondrial pathway

The absorbance of each sample was measured at 490 nm using an automated ELISA reader (SpectraMax M5, Molecular Devices, United States)